Effekte von Phosphorylierungen des humanen Herzmuskel-Troponin I und Mutationen im Troponin I-Gen auf die Regulation der Aktomyosin-Interaktion
- Effects of PKA-dependent phosphorylation of human cardiac troponin I (cTn) on the Ca\(^{2+}\)-regulation of actin-myosin interaction was studied with wild type cTnI and mutant cTnI with altered phosphorylation sites, cTnI-S22A and cTnI-S23A. fHCM-linked cTnI mutants, R145G, G203S and K206Q, were investigated in defined phosphorylation state as well. ActoS1-ATPase and \(\textit {in vitro}\) motility assays were performed on reconstituted thin filaments containing different cTnI species. The results show phosphorylation of cTnI by PKA causes two effects: mono- or bis-phosphorylation reduces the maximum Ca\(^{2+}\) activated actoS1-ATPase activity as well as the maximum Ca\(^{2+}\) activated mean sliding velocity of reconstituted thin filaments, bis-phosphorylation also causes a rightward shift of the Ca\(^{2+}\) activation curves of actoS1-ATPase activity and sliding filament velocity. The three fHCM-causing cTnI mutations exert different effects on the actomyosin system in response to phosphorylation of the serines 22 and 23.
Author: | Yi DengGND |
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URN: | urn:nbn:de:hbz:294-6905 |
Referee: | Rolf ThieleczekGND, Manfred HauserGND |
Document Type: | Doctoral Thesis |
Language: | English |
Date of Publication (online): | 2003/03/21 |
Date of first Publication: | 2003/03/21 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Granting Institution: | Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie |
Date of final exam: | 2002/12/04 |
Creating Corporation: | Fakultät für Biologie und Biotechnologie |
GND-Keyword: | Troponin; Actin; Myosin; Phosphorylierung; Herzmuskel |
Institutes/Facilities: | Institut für Physiologische Chemie, Abteilung Biochemie supramolekularer Systeme |
Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
faculties: | Fakultät für Biologie und Biotechnologie |
Licence (German): | Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |