ATR-FTIR spectroscopy of membrane-bound Ras protein
- The first high quality Amide I bands of membrane-bound Ras were obtained after establishing an ATR-FTIR set-up. Protein secondary structure analyses were performed by an improved curve-fitting technique. Truncated H-Ras (1-166) and membrane-bound N-Ras (1-188) protein secondary structures were in accordance with the literature. Tyr side chain band splitting was observed for the membrane-bound N-Ras. Besides, secondary structure deviations were apparent, when there was no membrane available to the protein for binding. Measurements were also performed with the polarized light. The study of membrane-bound Ras protein interaction with the nitric oxide was initiated in a model system. Protein activity was attained. The lipid absorption intensity was modulated. In relation, random to sheet transitions of the membrane-bound Ras protein was observed. Stabilizing interactions of the lipid C=O region were accompanying. This could be associated with the conformational changes in the Ras protein.
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| Author: | Yekbun AdigüzelGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-22478 |
| Title Additional (German): | ATR-FTIR Spektroskopie von membran-gebundenem Ras Protein |
| Referee: | Klaus GerwertORCiDGND, Hanns HattGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2008/06/12 |
| Date of first Publication: | 2008/06/12 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie |
| Date of final exam: | 2008/05/21 |
| Creating Corporation: | Fakultät für Biologie und Biotechnologie |
| GND-Keyword: | Ras-Proteine; Membran; Sekundärstruktur; FT-IR-Spektroskopie; Stickstoffmonoxid |
| Institutes/Facilities: | Lehrstuhl für Biophysik |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| faculties: | Fakultät für Biologie und Biotechnologie |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
