Evaluation of monomeric and trimeric PS1 in native systems and in semiartificial systems for biohydrogen production
Evaluierung von monomerem und trimerem Photosystem 1 in nativen Systemen sowie in semiartifiziellen Systemen zur Biowasserstofferzeugung
- Purification of highly active trimeric PS1 was optimized and speeded up from 32 to 15 hours, and new and mild methods were developed for the qualitative and quantitative production of monomeric PS1. This enabled for a detailed analysis in comparison with the trimeric form in terms of pigment/cofactor contents, subunit composition, photosynthetic electron transport and photocurrent of immobilized PS1. Both complexes showed almost identical spectroscopic characteristics of LD-spectra, redox potential, Chl \(\it a\)P700 ratio, carotenoid contents, subunit composition and activity. Modeling of kinetics measurements (time resolved fluorescence in the lower ps range) indicated the same origin of the long wavelength chlorophylls (LWCs) in both complexes. Optimization of photocurrent measurements of PS1, which had been immobilized on a gold surfaces by a \(\textit {His-tag}\), showed the higher efficiency of the artificial electron donor PMS in comparison with the native Cyt\( _{C6}\).
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| Author: | Eithar Abdel Rashid El-MohsnawyGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-20186 |
| Referee: | Matthias RögnerGND, Mathias LübbenGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2007/10/09 |
| Date of first Publication: | 2007/10/09 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie |
| Date of final exam: | 2007/08/30 |
| Creating Corporation: | Fakultät für Biologie und Biotechnologie |
| GND-Keyword: | Photosystem; Zirkulardichroismus; Lineardichroismus; Photostrom; Photostabilität |
| Institutes/Facilities: | Lehrstuhl für Biochemie der Pflanzen |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| faculties: | Fakultät für Biologie und Biotechnologie |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
