Functional investigation of odorant receptor in non-olfactory tissues

Besides their expression in the cilia of olfactory sensory neurons, ORs are also expressed in non-olfactory tissues. However, in non-olfactory tissues, OR function remains enigmatic. In the present study, we discovered novel functions of olfactory receptors in non-olfactory tissues. A human OR named "prostate specific G-protein coupled receptor" was cloned and characterized. The identified ligands induce a Ca\(^{2+}\) influx in prostate cancer epithelial cells. Ligand treatment resulted in inhibition of prostate cell proliferation via activation of MAPKs. OR51E2 was found to be functionally expressed in human testis. Activation of OR51E2 elicits phospholipase C mediated Ca\(^{2+}\) release from internal stores, and enhances sperm capacitation. MOR283-2 was found to function as chemosensory receptor in immature spermatogenic cells. Amylbutyrate was identified as the ligand for MOR283-2. Activation of MOR283-2 leads to an enhancement of differentiation and survival of spermatogenic cell.

Metadaten
Author:	Weiyi Zhang
URN:	urn:nbn:de:hbz:294-20237
Referee:	Hanns Hatt GND, Eva Neuhaus GND
Document Type:	Doctoral Thesis
Language:	English
Date of Publication (online):	2007/10/18
Date of first Publication:	2007/10/18
Publishing Institution:	Ruhr-Universität Bochum, Universitätsbibliothek
Granting Institution:	Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie
Date of final exam:	2007/09/21
Creating Corporation:	Fakultät für Biologie und Biotechnologie
GND-Keyword:	Geruchssinn / Rezeptor; Ligand (Biochemie); Geruchswahrnehmung; Prostata; Spermium
Dewey Decimal Classification:	Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie
Licence (German):	Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht

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