The fungal phytochrome FphA from \(\textit {Aspergillus nidulans}\)
Das pilzliche Phytochrom FphA aus Aspergillus nidulans
- The red-light photoreceptor FphA from \(\textit {Aspergillus nidulans}\) is involved in the regulation of developmental processes in response to light. The recombinant photosensory domain FphAN753 displays all features of a bonafide phytochrome. It covalently binds BV as chromophore, and undergoes red/far-red light inducible photoconversion with both parent states being protonated. The N-terminal variable extension stabilizes the active Pfr state. Deletion of the conserved H504, involved in the hydrogen-bonding network of the protein moiety and chromophore, impaired BV attachment and photoreversibility. Moreover, it was confirmed that FphA is a functional sensor histidine kinase with a strong red-light dependent autophosphorylation. In addition, \(\it {trans}\)-phosphorylation activity to a second monomer occurred which was bilin-independently. It was also observed that hetero- and homo-oligomerization of FphA and FphA variants led to enhanced autophosphorylation including the "inactive" Pr form.
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| Author: | Sonja BrandtGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-23819 |
| Referee: | Nicole FrankenbergGND, Eckhard HofmannORCiDGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2008/11/18 |
| Date of first Publication: | 2008/11/18 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie |
| Date of final exam: | 2008/10/09 |
| Creating Corporation: | Fakultät für Biologie und Biotechnologie |
| GND-Keyword: | Photorezeptor; Tetrapyrrole; Protein-Histidin-Kinasen; Phosphorylierung; Biliverdin |
| Institutes/Facilities: | Lehrstuhl für Biologie der Mikroorganismen, Arbeitsgruppe Physiologie der Mikroorganismen |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Pflanzen (Botanik) |
| faculties: | Fakultät für Biologie und Biotechnologie |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
