Mutational, structural and dynamic analysis of the Ran-RanBP2 interaction in nucleo-cytoplasmic transport
- The functions of some conserved motifs of Ran and Ran binding domain 1 and 2 of RanBP2 were characterized by the kinetics and equilibrium of spectroscopy using analogues of guanine nucleotides, Isothermal Titration Calorimetry (ITC) and structural analysis (crystallization and NMR) approaches. The results suggest 1) The C-terminal DEDDDL motif destabilizes GTP complexation with Ran. This tail of Ran is important for binding to BD1. 2) The N-terminal fragment of BD1 contributes largely to the recognition of Ran in its GTP form. 3) The conserved \(^{57}\)WKER motif of BD1 stabilizes GTP binding on Ran by interactions with the effector loop of Ran. 4) BD1 binds to Ran in its GDP form with a weak affinity of 50 \(\mu\)M. It promotes the dissociation rate of GDP from the Ran*GDP complex putatively by deformation of \(\alpha\)\(\tiny{1b}\) and \(\alpha\)\(\tiny{1a}\) of Ran in the GDP form. 5) NMR studies reveal that there is an extra 5\(^{th}\)-\(\beta\) strand in the core domain of BD2 in solution, which make BD2 a perfect canonical PH domain.
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| Author: | Xiaodong Zhao |
|---|---|
| URN: | urn:nbn:de:hbz:294-5632 |
| Referee: | Andrea BlöchlGND, Wolfram SanderORCiDGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2003/03/18 |
| Date of first Publication: | 2003/03/18 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, Fakultät für Chemie und Biochemie |
| Date of final exam: | 2002/07/16 |
| Creating Corporation: | Fakultät für Chemie und Biochemie |
| GND-Keyword: | Transferreaktion; NMR-Spektroskopie; Guanosintriphosphat; Kinetik; Chemische Bindung |
| Institutes/Facilities: | Max-Planck-Institut für molekulare Physiologie, Dortmund, Abteilung "Strukturelle Biologie" |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
