Molecular quantification of viruses in surface water
Molekulare Quantifizierung von Viren in Oberflächenwasser
- Viruses are a major cause of waterborne and water related diseases. A few viral particles can be found in surface water, so enrichment steps are required prior to the analysis of water samples. The virus adsorption elution method was optimized for enterovirus (EV), human adenovirus (HAdV), human polyomavirus (HPyV), norovirus (NoV) and somatic coliphages (SOMCPH). The results revealed that high salt-alkaline solution of phosphate buffer with 0.1%Trition X-100 is the buffer of choice for simultaneous recovery of viruses under study. Since it is not practical to monitor all viruses that may contaminate water, virus indicator of water contamination is needed. Accordingly, different viruses were proposed as indicators of water contamination such as EV, HAdV, HPyV, human bocavirus, NoV, rotavirus, Torque teno virus, human picobirnavirus, pepper mild mottle virus (PMMoV) and SOMCPH. This work showed that HAdV, HPyV and PMMoV could be promising indicators of surface water contamination.
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| Author: | Ibrahim A. Hamza EwessGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-30908 |
| Subtitle (English): | development of indicator of viral contamination in water |
| Subtitle (German): | Entwicklung eines Indikators zur Bestimmung der viralen Kontamination in Wasser |
| Referee: | Michael WilhelmORCiDGND, Franz NarberhausORCiDGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2011/02/28 |
| Date of first Publication: | 2011/02/28 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, Fakultät für Biologie und Biotechnologie |
| Date of final exam: | 2010/12/22 |
| Creating Corporation: | Fakultät für Biologie und Biotechnologie |
| GND-Keyword: | Viren; Oberflächenwasser; Kontamination; Indikator; Wasserprobe |
| Institutes/Facilities: | Fakultät für Biologie und Biotechnologie, Abteilung für Hygiene, Sozial- und Umweltmedizin |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| faculties: | Fakultät für Biologie und Biotechnologie |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
