Kombinatorische Festphasensynthese neuer kationischer Lipide und High-Throughput-Screening ihrer Transfektionseigenschaften
- Aim of the project was the development and application of suitable technologies for the multiple and combinatorial solid phase synthesis of transfections lipids. The strategy was based on the utilisation of trityl and vinyl sulfone resins as the solid support. Lipid libraries were synthesized and their transfection properties were screened. This strategy allowed to generate a diversity of new types of lipids whose structures were broadly varied. Severals new classes of transfection lipids were discovered by high throuput screening. The best members are 15 times more efficient than the well known cationic lipid DOTAP while having comparable toxicities.
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| Author: | Karl Christian LenßenGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-7445 |
| Referee: | Günter von KiedrowskiGND, Martin FeigelGND, Bernhard HovemannGND |
| Document Type: | Doctoral Thesis |
| Language: | German |
| Date of Publication (online): | 2003/05/27 |
| Date of first Publication: | 2003/05/27 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, Fakultät für Chemie und Biochemie |
| Date of final exam: | 2002/12/17 |
| Creating Corporation: | Fakultät für Chemie und Biochemie |
| GND-Keyword: | Transfektion; Lipide; Kombinatorische Synthese; High throughput screening; Gentransfer |
| Institutes/Facilities: | Lehrstuhl für Organische Chemie I |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Chemie, Kristallographie, Mineralogie |
| faculties: | Fakultät für Chemie und Biochemie |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
