ABCC6 - a new player in cellular cholesterol and lipoprotein metabolism?
- \(\textbf{Background:}\) Dysregulations in cholesterol and lipid metabolism have been linked to human diseases like hypercholesterolemia, atherosclerosis or the metabolic syndrome. Many ABC transporters are involved in trafficking of metabolites derived from these pathways. Pseudoxanthoma elasticum (PXE), an autosomal-recessive disease caused by ABCC6 mutations, is characterized by atherogenesis and soft tissue calcification. \(\textbf{Methods:}\) In this study we investigated the regulation of cholesterol biosynthesis in human dermal fibroblasts from PXE patients and healthy controls. \(\textbf{Results:}\) Gene expression analysis of 84 targets indicated dysregulations in cholesterol metabolism in PXE fibroblasts. Transcript levels of ABCC6 were strongly increased in lipoprotein-deficient serum (LPDS) and under serum starvation in healthy controls. For the first time, increased HMG CoA reductase activities were found in PXE fibroblasts. We further observed strongly elevated transcript and protein levels for the proprotein convertase subtilisin/kexin type 9 (PCSK9), as well as a significant reduction in APOE mRNA expression in PXE. \(\textbf{Conclusion:}\) Increased cholesterol biosynthesis, elevated PCSK9 levels and reduced APOE mRNA expression newly found in PXE fibroblasts could enforce atherogenesis and cardiovascular risk in PXE patients. Moreover, the increase in ABCC6 expression accompanied by the induction of cholesterol biosynthesis supposes a functional role for ABCC6 in human lipoprotein and cholesterol homeostasis.
Author: | Patricia KuzajGND, Joachim KuhnGND, Mareike Dabisch-RutheGND, Isabel FaustORCiDGND, Christian GöttingGND, Cornelius KnabbeGND, Doris HendigORCiDGND |
---|---|
URN: | urn:nbn:de:hbz:294-57475 |
DOI: | https://doi.org/10.1186/1476-511X-13-118 |
Parent Title (English): | Lipids in health and disease |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2018/06/25 |
Date of first Publication: | 2014/07/27 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | APOE; LDLR; Open Access Fonds; PCSK9; SREBP2 ABC transporter; ABCC6; Atherosclerosis; Cholesterol biosynthesis; HMG CoA reductase; Pseudoxanthoma elasticum |
Volume: | 13 |
First Page: | 118-1 |
Last Page: | 118-17 |
Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of the Ruhr-Universität Bochum. |
Institutes/Facilities: | Herz- und Diabeteszentrum NRW, Institut für Laboratoriums- und Transfusionsmedizin |
Dewey Decimal Classification: | Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit |
open_access (DINI-Set): | open_access |
faculties: | Medizinische Fakultät |
Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |