Two distinct cardiolipin synthases operate in \(\textit {Agrobacterium tumefaciens}\)

  • Cardiolipin (CL) is a universal component of energy generating membranes. In most bacteria, it is synthesized via the condensation of two molecules phosphatidylglycerol (PG) by phospholipase D-type cardiolipin synthases (PLD-type Cls). In the plant pathogen and natural genetic engineer \(\textit {Agrobacterium tumefaciens}\) CL comprises up to 15% of all phospholipids in late stationary growth phase. \(\textit {A. tumefaciens}\) harbors two genes, \(\it atu1630\) (\(\it cls1\)) and \(\it atu2486\) (\(\it cls2\)), coding for PLD-type Cls. Heterologous expression of either \(\it cls1\) or \(\it cls2\) in \(\textit {Escherichia coli}\) resulted in accumulation of CL supporting involvement of their products in CL synthesis. Expression of \(\it cls1\) and \(\it cls2\) in \(\textit {A. tumefaciens}\) is constitutive and irrespective of the growth phase. Membrane lipid profiling of \(\textit {A. tumefaciens}\) mutants suggested that Cls2 is required for CL synthesis at early exponential growth whereas both Cls equally contribute to CL production at later growth stages. Contrary to many bacteria, which suffer from CL depletion, \(\textit {A. tumefaciens}\) tolerates large changes in CL content since the CL-deficient \(\it cls1/cls2\) double mutant showed no apparent defects in growth, stress tolerance, motility, biofilm formation, UV-stress and tumor formation on plants.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Metadaten
Author:Simon CzolkossGND, Christiane FritzGND, Georg HölzlGND, Meriyem AktasORCiDGND
URN:urn:nbn:de:hbz:294-58412
DOI:https://doi.org/10.1371/journal.pone.0160373
Parent Title (English):PLoS one
Document Type:Article
Language:English
Date of Publication (online):2018/07/04
Date of first Publication:2016/07/29
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:Open Access Fonds
Volume:11
Issue:7
First Page:e0160373-1
Last Page:e0160373-18
Note:
Article Processing Charge funded by the Open Access Publication Fund of Ruhr-Universität Bochum.
Note:
PLoS ONE, Bd. 11.2016, H. 7, Artikelnummer e0160373
Institutes/Facilities:Lehrstuhl für Biologie der Mikroorganismen
Dewey Decimal Classification:Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie
open_access (DINI-Set):open_access
faculties:Fakultät für Biologie und Biotechnologie
Licence (English):License LogoCreative Commons - CC BY 4.0 - Attribution 4.0 International