The CCK-B/gastrin receptor splice variant retaining intron 4 sequence mediates transcriptional activation of the cyclooxygenase-2 promoter in human colorectal cancer cells and in COS-7 cells
- Problem: The expression of the human cholecystokinin-B/gastrin receptor has been reported in human colorectal cancers. Recently, a novel gastrin receptor has been cloned. The purpose of the present study was to determine whether the novel gastrin receptor transactiviates COX-2 promoter in human colon cancer cells and in COS-7 cells. Methods: Both cells were transfected with wild type and novel gastrin captors. Stimulated with gastrin-17, transactivation of COX-2 promoter was determined by luciferase reporter gene assay. Induction of COX-2 expression was explored at the mRNA and protein level using real time RT-PCR and Western blotting. Prostaglandin E2 secretion was measured by ELISA. Results: Gastrin transactivates the COX-2 promoter in both cells. Three pathways are required for the transactivation of COX-2. Gastrin increased mRNA expression and stimulated PGE2 secretion. Discussion: The novel gastrin receptor transactiviates COX-2 and is thus likely to confer oncogenic properties.
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| Author: | He HuangGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-16718 |
| Referee: | Frank SchmitzORCiDGND, Martin PfohlGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2006/07/24 |
| Date of first Publication: | 2006/07/24 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, Medizinische Fakultät |
| Date of final exam: | 2006/06/29 |
| Creating Corporation: | Medizinische Fakultät |
| GND-Keyword: | Gastrin; Darmkrebs; Rezeptor; Cholecyctokinin; Tumor |
| Institutes/Facilities: | St. Josef-Hospital Bochum, Medizinische Klinik I |
| Dewey Decimal Classification: | Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit |
| faculties: | Medizinische Fakultät |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
