Electrochemical detection of nitric oxide from living cells

  • Two different types of NO-sensor were developed. The first type of NO-sensor developed was based on the electrochemical entrapment of Ni-TmPyP within a polymer matrix formed by anodic electrodeposition paint on the surface of Pt electrodes. The second type of NO-sensor was developed by polymerizing a layer of Ni-TSPc on a Pt surface followed by layer of Nafion\(^\circledR\). The chemical modifications on both types of electrode developed were successfully applied for the sensitive determination of nitric oxide. In the measurement of NO release from living cells, the positioning of the NO-sensor plays an important role. Hence, different procedures were developed for accurate and precise detection of NO. Simultaneous detection of NO and glutamate from cells at a known distance was successfully achieved. Finally, single cell measurement of NO release was achieved with the combination of a developed highly sensitive vibrational disk-shaped NO-sensor and constant distance mode SECM at a sub-\(\mu\)m distance.

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Author:Sonnur IsikGND
Referee:Wolfgang SchuhmannORCiDGND, William S. SheldrickGND
Document Type:Doctoral Thesis
Date of Publication (online):2006/05/04
Date of first Publication:2006/05/04
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Granting Institution:Ruhr-Universität Bochum, Fakultät für Chemie und Biochemie
Date of final exam:2006/02/03
Creating Corporation:Fakultät für Chemie und Biochemie
GND-Keyword:Rasterelektrochemisches Mikroskop; Biosensor; Elektrochemie; Glutamate; Neurotransmitter
Institutes/Facilities:Lehrstuhl für Analytische Chemie - Elektroanalytik & Sensorik
Dewey Decimal Classification:Naturwissenschaften und Mathematik / Chemie, Kristallographie, Mineralogie
faculties:Fakultät für Chemie und Biochemie
Licence (German):License LogoKeine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht