Intracellular accumulation of methylglyoxal by glyoxalase 1 knock down alters collagen homoeostasis in L6 myoblasts
- Hyperglycemia results in accumulation of the reactive dicarbonyl methylglyoxal (MG). Methylglyoxal is detoxified by the glyoxalase system (glyoxalase 1 and 2). The influence of glyoxalase 1 knockdown on expression of collagens 1, 3, 4, and 5 in L6 myoblasts under hyperglycemic conditions was investigated. Increased biosynthesis of collagens 1, 3, 4, and 5 was detected at mRNA-level following knockdown of glyoxalase 1 (GLO1). At the protein level a significant elevation of the concentration of collagen 1 and 4 was shown, whereas no increase of collagen 5 and a non-significant increase in collagen 3 were detectable. These results could partially explain MG-induced changes in the extracellular matrix (ECM) which account for increased fibrosis and impaired function in myocytes. The mechanisms by which reactive glucose metabolites influence ECM composition deserve further investigation.
| Author: | Bernd StratmannGND, Bernhard GoldsteinGND, Paul ThornalleyGND, Naila RabbaniGND, Diethelm TschöpeGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-70846 |
| DOI: | https://doi.org/10.3390/ijms18030480 |
| Parent Title (English): | International journal of molecular sciences |
| Publisher: | MDPI |
| Place of publication: | Basel |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2020/03/30 |
| Date of first Publication: | 2017/02/23 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Tag: | collagen; dicarbonyl proteome; glyoxalase 1; hyperglycemia; methylglyoxal |
| Volume: | 18 |
| Issue: | 3, Article 480 |
| First Page: | 480-1 |
| Last Page: | 480-10 |
| Institutes/Facilities: | Herz- und Diabeteszentrum NRW |
| open_access (DINI-Set): | open_access |
| faculties: | Medizinische Fakultät |
| Licence (English): |  Creative Commons - CC BY 4.0 - Attribution 4.0 International |
