Transfer of the experimental autoimmune glaucoma model from rats to mice

  • Studies have suggested an involvement of the immune system in glaucoma. Hence, a rat experimental autoimmune glaucoma model (EAG) was developed to investigate the role of the immune response. Here, we transferred this model into mice. Either 0.8 mg/mL of the optic nerve antigen homogenate (ONA; ONA 0.8) or 1.0 mg/mL ONA (ONA 1.0) were injected in 129/Sv mice. Controls received sodium chloride. Before and 6 weeks after immunization, the intraocular pressure (IOP) was measured. At 6 weeks, retinal neurons, glia cells, and synapses were analyzed via immunohistology and quantitative real-time PCR (RT-qPCR). Additionally, optic nerves were examined. The IOP stayed in the normal physiological range throughout the study (\(\it p\) > 0.05). A significant reduction of retinal ganglion cells (RGCs) was noted in both immunized groups (\(\it p\) < 0.001). Remodeling of glutamatergic and GABAergic synapses was seen in ONA 1.0 retinas. Furthermore, both ONA groups revealed optic nerve degeneration and macrogliosis (all: \(\it p\) < 0.001). An increase of activated microglia was noted in ONA retinas and optic nerves (\(\it p\) < 0.05). Both ONA concentrations led to RGC loss and optic nerve degeneration. Therefore, the EAG model was successfully transferred from rats to mice. In further studies, transgenic knockout mice can be used to investigate the pathomechanisms of glaucoma more precisely.

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Metadaten
Author:Sabrina ReinehrORCiDGND, Jacqueline ReinhardORCiDGND, Susanne WiemannGND, Karoline HesseGND, Christina VossGND, Marcel GandejGND, Burkhard DickORCiDGND, Andreas FaissnerORCiDGND, Stephanie Christine JoachimORCiDGND
URN:urn:nbn:de:hbz:294-71006
DOI:https://doi.org/10.3390/ijms20102563
Parent Title (English):International journal of molecular sciences
Subtitle (English):new options to study glaucoma disease
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2020/04/05
Date of first Publication:2019/05/24
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:Open Access Fonds
autoimmune glaucoma model; immunization; retinal ganglion cell loss; synapse
Volume:20
Issue:10, Article 2563
First Page:2563-1
Last Page:2563-19
Note:
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum.
Institutes/Facilities:Knappschaftskrankenhaus Bochum, Klinik für Augenheilkunde
open_access (DINI-Set):open_access
Licence (English):License LogoCreative Commons - CC BY 4.0 - Attribution 4.0 International