Nasal chemosensory-stimulation evoked activity patterns in the rat trigeminal ganglion visualized by \(\textit {In Vivo}\) voltage-sensitive dye imaging

  • Mammalian nasal chemosensation is predominantly mediated by two independent neuronal pathways, the olfactory and the trigeminal system. Within the early olfactory system, spatiotemporal responses of the olfactory bulb to various odorants have been mapped in great detail. In contrast, far less is known about the representation of volatile chemical stimuli at an early stage in the trigeminal system, the trigeminal ganglion (TG), which contains neurons directly projecting to the nasal cavity. We have established an \(\textit {in vivo}\) preparation that allows high-resolution imaging of neuronal population activity from a large region of the rat TG using voltage-sensitive dyes (VSDs). Application of different chemical stimuli to the nasal cavity elicited distinct, stimulus-category specific, spatiotemporal activation patterns that comprised activated as well as suppressed areas. Thus, our results provide the first direct insights into the spatial representation of nasal chemosensory information within the trigeminal ganglion imaged at high temporal resolution.

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Metadaten
Author:Markus RothermelGND, Shien Wei Benedict NgGND, Agnieszka Grabska-BarwińskaGND, Hanns HattGND, Dirk JanckeORCiDGND
URN:urn:nbn:de:hbz:294-71589
DOI:https://doi.org/10.1371/journal.pone.0026158
Parent Title (English):PLoS ONE
Publisher:Public Library of Science
Place of publication:San Francisco
Document Type:Article
Language:English
Date of Publication (online):2020/05/14
Date of first Publication:2011/10/19
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:Action potentials; Electrophysiology; Ethanol; Ganglia; In vivo imaging; Nasal cavity; Neuroimaging; Neurons
Volume:6
Issue:10, Artikel e26158
First Page:e26158-1
Last Page:e26158-9
Institutes/Facilities:Lehrstuhl für Zellphysiologie
Research Department of Neuroscience
Protein Research Department
open_access (DINI-Set):open_access
Licence (English):License LogoCreative Commons - CC BY 4.0 - Attribution 4.0 International