Christoph Rumancev, Andreas Gräfenstein, Tobias Vöpel, Susan Maria Marta Stuhr, Andreas Robert von Gundlach, Tobias Senkbeil, Jan Garrevoet, L Jolmes, Benedikt König, Simon Ebbinghaus, Axel Rosenhahn
- A new Rococo 2 X-ray fluorescence detector was implemented into the cryogenic sample environment at the Hard X-ray Micro/Nano-Probe beamline P06 at PETRA III, DESY, Hamburg, Germany. A four sensor-field cloverleaf design is optimized for the investigation of planar samples and operates in a backscattering geometry resulting in a large solid angle of up to 1.1 steradian. The detector, coupled with the Xspress 3 pulse processor, enables measurements at high count rates of up to 10\(^{6}\) counts per second per sensor. The measured energy resolution of ∼129 eV (Mn \(\Kappa\alpha\) at 10000 counts s\(^{−1}\)) is only minimally impaired at the highest count rates. The resulting high detection sensitivity allows for an accurate determination of trace element distributions such as in thin frozen hydrated biological specimens. First proof-of-principle measurements using continuous-movement 2D scans of frozen hydrated HeLa cells as a model system are reported to demonstrate the potential of the new detection system.
Metadaten| Author: | Christoph RumancevORCiDGND, Andreas GräfensteinGND, Tobias VöpelORCiDGND, Susan Maria Marta StuhrGND, Andreas Robert von GundlachGND, Tobias SenkbeilGND, Jan GarrevoetORCiDGND, L Jolmes, Benedikt KönigGND, Simon EbbinghausORCiDGND, Axel RosenhahnORCiDGND |
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| URN: | urn:nbn:de:hbz:294-71858 |
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| DOI: | https://doi.org/10.1107/s1600577519014048 |
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| Parent Title (English): | Journal of synchrotron radiation |
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| Publisher: | Wiley-VCH Verlag |
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| Place of publication: | Weinheim |
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| Document Type: | Article |
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| Language: | English |
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| Date of Publication (online): | 2020/05/26 |
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| Date of first Publication: | 2020/01/01 |
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| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
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| Tag: | HeLa cells; SDD; XRF; cryogenic; solid angle |
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| Volume: | 27 |
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| Issue: | 1 |
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| First Page: | 60 |
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| Last Page: | 66 |
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| Note: | Dieser Beitrag ist auf Grund des DEAL-Wiley-Vertrages frei zugänglich. |
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| Institutes/Facilities: | Lehrstuhl für Physikalische Chemie II |
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| Arbeitsgruppe Analytische Chemie - Biointerfaces |
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| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Chemie, Kristallographie, Mineralogie |
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| open_access (DINI-Set): | open_access |
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| Licence (English): |  Creative Commons - CC BY 4.0 - Attribution 4.0 International |
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