Nydus One Syringe Extruder (NOSE)

  • Bioprinting, combined with other tissue engineering techniques, is a promising method to create engineered tissues or standardized 3D cell culturing models. It has potential applications in the development of animal free models for drug toxicity screenings or for personalized medicine approaches. Here we report the conversion of the worldwide used open source 3D printer (RepRap, Prusa i3) to a functional and affordable bioprinter by substituting the common plastic-extruder with the \(\bf N\)ydus \(\bf O\)ne \(\bf S\)yringe \(\bf E\)xtruder (\(\bf NOSE\)). The \(\bf NOSE\) modification enables mechanical hydrogel extrusion as well as a tunable deposition precision and volume by featuring a modular syringe-holder concept. A cost effective and simple hardware design including a custom software (termed ‘composer’) was developed to prove that this technique can be made accessible for a broader public. Furthermore, the printing procedure was optimized for the peer-reviewed FRESH-method by Hinton et al. which allows to print geometrical complex cell-laden constructs. We provide a detailed protocol on the creation of the FRESH-gel to ensure the reproducibility of this state of the art method. As a proof of concept HEK293 cells as well as mouse embryonic stem cells (mESC) were utilized for cell-laden printing. Depending on the cellular source the survival rates range from 60% up to 95%. Automatized quantitative viability analysis was performed using the open source image analysis tool Icy, in particular the “spot detector” plugin. A detailed protocol allows the recreation of the assay. Further data utilizing a support bath printing technique reveal limitations regarding the printing and residential time of cell-laden constructs.

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Metadaten
Author:Nils BesslerGND, Dennis OgiermannORCiDGND, Maj-Britt BuchholzGND, Alexander SantelGND, Jan HeidenreichGND, Rawas AhmmedGND, Holm ZaehresORCiDGND, Beate Brand-SaberiORCiDGND
URN:urn:nbn:de:hbz:294-73085
DOI:https://doi.org/10.1016/j.ohx.2019.e00069
Parent Title (English):HardwareX
Subtitle (English):A Prusa i3 3D printer conversion for bioprinting applications utilizing the FRESH-method
Publisher:Elsevier B.V.
Place of publication:Amsterdam
Document Type:Article
Language:English
Date of Publication (online):2020/07/09
Date of first Publication:2019/10/01
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:Open Access Fonds
Bioprinting Protocol; Bioprinting Software; Mechanical Syringe Extruder; Open Source Bioprinter; RepRap Modificiation
Volume:6
Issue:Artikel e00069
First Page:e00069-1
Last Page:e00069-19
Note:
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum.
Institutes/Facilities:Institut für Anatomie, Abteilung für Anatomie und Molekulare Embryologie
Dewey Decimal Classification:Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit
open_access (DINI-Set):open_access
Licence (English):License LogoCreative Commons - CC BY-NC-ND 4.0 - Attribution-NonCommercial-NoDerivatives 4.0 International