Caffeine and \(NAD^{+}\) improve motor neural integrity of dissociated wobbler cells in vitro

  • Amyotrophic lateral sclerosis (ALS) is a common degenerative disease of the central nervous system concerning a progressive loss of upper and lower motor neurons. While 5%–10% of patients are diagnosed with the inherited form of the disease, the vast majority of patients suffer from the less characterized sporadic form of ALS (sALS). As the wobbler mouse and the ALS show striking similarities in view of phenotypical attributes, the mouse is rated as an animal model for the disease. Recent investigations show the importance of nicotinamide adenine dinucleotide (\(NAD^{+}\)) and its producing enzyme nicotinic acid mononucleotide transferase 2 (Nmnat2) for neurodegeneration as well as for the preservation of health of the neuronal cells. Furthermore, it is newly determined that these molecules show significant downregulations in the spinal cord of wobbler mice in the stable phase of disease development. Here, we were able to prove a positive benefit on affected motor neurons from an additional \(NAD^{+}\) supply as well as an increase in the Nmnat2 level through caffeine treatment in cells in vitro. In addition, first assumptions about the importance of endogenous and exogenous factors that have an influence on the wellbeing of motor nerve cells in the model of ALS can be considered.

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Metadaten
Author:Mareike ZwillingGND, Carsten TheißORCiDGND, Veronika MatschkeORCiDGND
URN:urn:nbn:de:hbz:294-74470
DOI:https://doi.org/10.3390/antiox9060460
Parent Title (English):Antioxidants
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2020/08/13
Date of first Publication:2020/05/27
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:Open Access Fonds
ALS; Nmnat2; ROS; \(NAD^{+}\) precursor; amyotrophic lateral sclerosis; degeneration; motor neuron disease; neuroprotection
Volume:9
Issue:6, Article 460
First Page:460-1
Last Page:460-15
Note:
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum.
Institutes/Facilities:Institut für Anatomie, Abteilung für Cytologie
Lehrstuhl für Biochemie I, Rezeptorbiochemie
open_access (DINI-Set):open_access
faculties:Medizinische Fakultät
Licence (English):License LogoCreative Commons - CC BY 4.0 - Attribution 4.0 International