Nina Babel, Moritz Anft, Arturo Blazquez-Navarro, Adrian Doevelaar, Felix Sebastian Seibert, Frederic Bauer, Benjamin Johannes Rohn, Bodo Hoelzer, Constantin J. Thieme, Toralf Roch, Toni Luise Meister, Stephanie Pfänder, Eike Steinmann, Ulf Dittmer, Peter Schenker, Kerstin Amann, Richard Viebahn, Ulrik Stervbo-Kristensen, Timm Westhoff
- The optimal management in transplant recipients with coronavirus disease 2019 (COVID-19) remains uncertain. The main concern is the ability of immunosuppressed patients to generate sufficient immunity for antiviral protection. Here, we report on immune monitoring facilitating a successful outcome of severe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-associated pneumonia, meningoencephalitis, gastroenteritis, and acute kidney and pancreas graft failure in a pancreas-kidney transplant recipient. Despite the very low numbers of circulating B, NK, and T cells identified in follow-up, a strong SARS-CoV-2 reactive T cell response was observed. Importantly, we detected T cells reactive to Spike, Membrane, and Nucleocapsid proteins of SARS-CoV-2 with majority of T cells showing polyfunctional proinflammatory Th1 phenotype at all analyzed time points. Antibodies against Spike protein were also detected with increasing titers in follow-up. Neutralization tests confirmed their antiviral protection. A correlation between cellular and humoral immunity was observed underscoring the specificity of demonstrated data. We conclude that analyzing the kinetics of nonspecific and SARS-CoV-2-reactive cellular and humoral immunity can facilitate the clinical decision on immunosuppression adjustment and allow successful outcome as demonstrated in the current clinical case. Although the antiviral protection of the detected SARS-CoV-2-reactive T cells requires further evaluation, our data prove an ability mounting a strong SARS-CoV-2-reactive T cell response with functional capacity in immunosuppressed patients.
MetadatenAuthor: | Nina BabelORCiDGND, Moritz AnftGND, Arturo Blazquez-NavarroGND, Adrian DoevelaarORCiDGND, Felix Sebastian SeibertORCiDGND, Frederic BauerGND, Benjamin Johannes RohnGND, Bodo HoelzerGND, Constantin J. ThiemeORCiDGND, Toralf RochORCiDGND, Toni Luise MeisterORCiDGND, Stephanie PfänderORCiDGND, Eike SteinmannORCiDGND, Ulf DittmerGND, Peter SchenkerORCiDGND, Kerstin AmannGND, Richard ViebahnORCiDGND, Ulrik Stervbo-KristensenORCiDGND, Timm WesthoffORCiDGND |
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URN: | urn:nbn:de:hbz:294-99303 |
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DOI: | https://doi.org/10.1111/ajt.16252 |
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Parent Title (English): | American journal of transplantation |
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Publisher: | Wiley |
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Place of publication: | Hoboken, New Jersey |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2023/05/30 |
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Date of first Publication: | 2020/08/10 |
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Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
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Tag: | T cell biology; basic (laboratory) research; cellular biology; complication; immune modulation; immunosuppression; infection and infectious agents – viral; infectious; kidney transplantation; nephrology; science; translational research |
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Volume: | 20 |
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Issue: | 11 |
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First Page: | 3210 |
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Last Page: | 3215 |
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Note: | Dieser Beitrag ist auf Grund des DEAL-Wiley-Vertrages frei zugänglich. |
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Institutes/Facilities: | Marienhospital Herne, Medizinische Klinik I |
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| Marienhospital Herne, Centrum für Translationale Medizin |
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Dewey Decimal Classification: | Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit |
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open_access (DINI-Set): | open_access |
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Licence (English): | Creative Commons - CC BY-NC 4.0 - Attribution-NonCommercial 4.0 International |
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